Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Clinicopathological Characteristics of the Study Population

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The levels of serum AFP, GPC3, GP73 and DCP in each subgroup. ( A ) AFP. ( B ) GPC3. ( C ) GP73. ( D ) DCP. ( E ) Representative image of each subgroup. * P <0.05, ** P <0.01. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin; ns, no significance; int, intensity.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Virus, Infection

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Diagnosis of HCC (Including All HCC Patients)

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: Assessment of the diagnostic value of serum AFP, GPC3, GP73 and DCP in differentiating HBV-related HCC from controls. ( A ) All HCC vs LC, CHB, HC. ( B ) All HCC vs LC, CHB. ( C ) All HCC v s LC. ( D ) Very early and early stage HCC vs LC, CHB, HC. ( E ) Very early and early stage HCC vs LC, CHB. ( F ) Very early and early stage HCC vs LC. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Diagnostic Assay, Virus, Infection

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Early Diagnosis of HCC (Including the Very Early HCC)

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Very Early Diagnosis of HCC

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The value of serum AFP, GPC3, GP73 and DCP in the very early diagnosis of HBV-related HCC. ( A ) Very early stage HCC vs LC, CHB, HC. ( B ) Very early stage vs LC, CHB. ( C ) Very early stage vs LC. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Virus, Infection

Journal: bioRxiv

Article Title: A Common PD-Risk GBA1 Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction

doi: 10.1101/2025.08.28.672891

Figure Lengend Snippet: A Venn diagram showing overlap in the identity of proteins with significantly altered abundance in lysosomes isolated from GBA1 -p.E326K KI, GBA1 -p.L444P KI HEK293T, and GBA1 KO cells, relative to lysosomes isolated from WT cells. B Volcano plots showing differential abundance of proteins identified in Lyso-IP samples from indicated GBA1 variant or KO cells compared to WT cells. Proteins with significantly changed abundance (p < 0.05; absolute log 2 FC ≥ 0.5) are highlighted in red or blue for increased or decreased abundance, respectively. Proteins identified as mitochondrial in origin in the MitoCarta 3.0 database are highlighted in purple. C The number of proteins identified in each GBA1 variant or KO genotype belonging to the top ten most significant GO: Cell Component terms associated with the subset of significantly depleted proteins in E326K (vs WT) lysosomes. D Oxygen consumption rate (OCR) of HEK293T cells of indicated genotype, measured using Seahorse XF Cell Mito Stress Test assay. n = 3 independent experiments. E HEK293T cells of indicated genotype were stained with TMRM and MitoTracker Green, and the ratio of the sum (per nuclei) TMRM fluorescence to MitoTracker Green fluorescence, normalized to the mean of all WT cells, is plotted. n = 3 independent experiments. F Heatmap of the relative abundance of all identified glycosphingolipid hydrolases (related to the pathway shown in 2c) is shown as log 2 fold change (from WT mean) for both whole-cell and Lyso-IP samples from HEK293T cells of indicated genotype. Analytes that were not detected in a sample are shaded gray. G Representative immunoblot of Lyso-IP samples from HEK293T cells (of indicated genotype) expressing TMEM192-HA x3 tag. Immunoblots were probed with antibodies against GLA, GM2A, SGSH, and HA as a loading and lysosomal isolation control. H Quantification of lysosomal GLA levels (normalized per sample to HA band intensity), measured via immunoblotting of Lyso-IP fraction & normalized to mean of all WT samples. n = 3 independent experiments. Bar graphs in E and H show mean ± SEM with individual points representative of data from separate experimental replicates. All proteomics data (A-C, F) is from n = 3 independent experiments. Unless otherwise noted, all statistical tests were performed on genotype-level comparisons using robust linear model as described in methods and significance threshold was set at FDR adjusted P value < 0.05. Statistics in E and H were performed on genotype-level comparisons using one-way ANOVA with Dunnett’s multiple comparison test, where * = p < 0.0332, ** = p < 0.0021, **** = p < 0.0001.

Article Snippet: Primary antibodies used include: GCase (rb mAb, abcam, ab125065; 1/1000) β-actin (ms mAb, Sigma-Aldrich, A2228; 1/2000) HA-Tag (rb mAb, Cell Signaling Technology, #3724; 1/2000) NPC2 (rb pAb, Proteintech, 19888-1-AP; 1/1000) LIMP2 (rb mAb, Thermo, 703037; 1/1000) Strep-tag (ms mAb, Qiagen, 34850; 1/250) GLA (ms mAb, Proteintech, 66121-1-Ig; 1/1000) GM2A (rb mAb, abcam, ab313587; 1/1000) SGSH (rb mAb, abcam, ab200346; 1/1000) GCase (rb pAb, Sigma-Aldrich, G4171; 1/1000) phospho-Ubiquitin (Ser65) (rb mAb, Cell Signaling Technology, #62802; 1/1000) PINK1 (rb mAb, Cell Signaling Technology, #6946; 1/1000) TIM23 (ms mAb, BD Biosciences, 611222; 1/1000) PDHA1 (ms mAb, abcam, ab110330; 1/1000) Mitofilin (ms mAb, Proteintech, 68226-1-Ig; 1/1000)

Techniques: Isolation, Variant Assay, Staining, Fluorescence, Western Blot, Expressing, Control, Comparison

Journal: International Journal of Biological Sciences

Article Title: Septin4 promotes cell death in human colon cancer cells by interacting with BAX

doi: 10.7150/ijbs.44429

Figure Lengend Snippet: Septin4 participated in DOX-induced apoptosis of HCT116 cells. A. HCT116 cells were treated with DOX at 0, 0.005, 0.05, 0.2, 0.4, and 2 μmol/L for 48 h, to detect the expression of cleaved-caspase3 and cleaved-PARP1. B. Quantification of protein expression in A were shown as the means±S.D., *** P <0.001 C. The expression of PCNA and Septin4 was detected in the presence of DOX at 0, 0.005, 0.05, 0.2, 0.4, and 2 μmol/L for 48 h. D. Quantification of protein expression in C were shown as the means±S.D., ** P <0.01, *** P <0.001. E. The generation of ROS was detected by DHR staining under the treatment of 0, 0.005, 0.05, 0.2, 0.4, and 2 μmol/L DOX for 48 h. Scale bars, 100 μm. F. Quantitative analyses of the fluorescence intensity in E, data are shown as means±S.D., *** P <0.001. G. Statistical results of CCK8 analysis of HCT116 treated with DOX at 0, 0.005, 0.05, 0.2, 0.4, and 2 μmol/L for 48 h, data are shown as means±S.D., *** P <0.001.

Article Snippet: The membranes were incubated with blocking solution (TBST + 5% BSA) at room temperature for 1 h, then specific primary antibodies: goat polyclonal anti-Septin4 (ab166788, Abcam, UK), rabbit polyclonal anti-cleaved-caspase3 (19877-1-AP, Proteintech, USA), rabbit polyclonal anti-cleaved-PARP1 (5625S, Cell Signaling Technology, USA), rabbit polyclonal anti-BAX (50599-2-lg, Proteintech, USA), rabbit polyclonal anti-PCNA (10205-2-AP, Proteintech, USA), mouse monoclonal anti-Flag (GNI4110-FG, GNI, Japan), mouse monoclonal anti-GAPDH (10494-1-AP, Proteintech) or mouse monoclonal anti-β-actin (mAbcam 8226, Abcam, UK) were added and the blots were slowly shaken overnight at 4°C.

Techniques: Expressing, Staining, Fluorescence

Journal: International Journal of Biological Sciences

Article Title: Septin4 promotes cell death in human colon cancer cells by interacting with BAX

doi: 10.7150/ijbs.44429

Figure Lengend Snippet: Overexpression of Septin4 enhanced sensitivity to DOX in colon cancer cells. A. HCT116 overexpressing Flag-Vector and Flag-Septin4 were treated with DOX at a concentration of 0.05 μmol/L for 48 h to detect the expression of cleaved-PARP1 and Flag-Septin4. B. Quantitative analysis of the expression of cleaved-PARP1 in A . Data were shown as the means±S.D., *** P <0.001. C. HCT116 overexpressing Flag-Vector and Flag-Septin4 were treated with DOX at a concentration of 0.05 μmol/L for 48 h to detect the expression of cleaved-caspase3 and Flag-Septin4. D. Quantitative analysis of the expression of cleaved-caspase3. Data were shown as the means±S.D., *** P <0.001. E. HCT116 overexpressing Flag-Vector and Flag-Septin4 were treated with DOX at a concentration of 0.05 μmol/L for 48 h to detect the expression of PCNA and Flag-Septin4. F. Quantitative analysis of the expression of PCNA in E . Data were shown as the means±S.D., *** P <0.001. G. The generation of ROS was detected by DHR staining in HCT116 overexpressing Flag-Vector and Flag-Septin4 treated with DOX at a concentration of 0.05 μmol/L for 48 h. Scale bars, 100 μm. H. Quantitative analyses of the fluorescence intensity in G . Data are shown as means±S.D., *** P <0.001. I. Statistical results of CCK8 assay of HCT116 overexpressing Flag-Vector and Flag-Septin4 under the DOX treatment at 0, 0.005, 0.05, 0.2, 0.4, and 2 μmol/L for 48 h. Data are shown as means±S.D., ** P <0.01, *** P <0.001.

Article Snippet: The membranes were incubated with blocking solution (TBST + 5% BSA) at room temperature for 1 h, then specific primary antibodies: goat polyclonal anti-Septin4 (ab166788, Abcam, UK), rabbit polyclonal anti-cleaved-caspase3 (19877-1-AP, Proteintech, USA), rabbit polyclonal anti-cleaved-PARP1 (5625S, Cell Signaling Technology, USA), rabbit polyclonal anti-BAX (50599-2-lg, Proteintech, USA), rabbit polyclonal anti-PCNA (10205-2-AP, Proteintech, USA), mouse monoclonal anti-Flag (GNI4110-FG, GNI, Japan), mouse monoclonal anti-GAPDH (10494-1-AP, Proteintech) or mouse monoclonal anti-β-actin (mAbcam 8226, Abcam, UK) were added and the blots were slowly shaken overnight at 4°C.

Techniques: Over Expression, Plasmid Preparation, Concentration Assay, Expressing, Staining, Fluorescence, CCK-8 Assay

Journal: International Journal of Biological Sciences

Article Title: Septin4 promotes cell death in human colon cancer cells by interacting with BAX

doi: 10.7150/ijbs.44429

Figure Lengend Snippet: Depletion of Septin4 resisted DOX-induced apoptosis in HCT116 cells. A. Expression of cleaved-PARP1 and cleaved-caspase3 in NC and Septin4-knockdown HCT116 cells were treated with DOX at 0.05 μmol/L for 48 h. B. Quantitative analysis of the expression of cleaved-PARP1 and cleaved-caspase3 in A . Data were shown as the means±S.D., *** P <0.001. C. ROS staining of the NC and shSeptin4 HCT116 cells were treated with DOX at 0.05 μmol/L for 48 h. Scale bars, 100 μm. D. Quantitative analyses of the fluorescence intensity in C . Data are shown as means±S.D., *** P <0.001. E. Statistical results of CCK8 analysis of NC and shSeptin4 HCT116 cells under 0.05 μmol/L DOX treatment for 48 h, *** P <0.001.

Article Snippet: The membranes were incubated with blocking solution (TBST + 5% BSA) at room temperature for 1 h, then specific primary antibodies: goat polyclonal anti-Septin4 (ab166788, Abcam, UK), rabbit polyclonal anti-cleaved-caspase3 (19877-1-AP, Proteintech, USA), rabbit polyclonal anti-cleaved-PARP1 (5625S, Cell Signaling Technology, USA), rabbit polyclonal anti-BAX (50599-2-lg, Proteintech, USA), rabbit polyclonal anti-PCNA (10205-2-AP, Proteintech, USA), mouse monoclonal anti-Flag (GNI4110-FG, GNI, Japan), mouse monoclonal anti-GAPDH (10494-1-AP, Proteintech) or mouse monoclonal anti-β-actin (mAbcam 8226, Abcam, UK) were added and the blots were slowly shaken overnight at 4°C.

Techniques: Expressing, Knockdown, Staining, Fluorescence